Lactobacillus MRS Agar

Description

Himedia Lactobacillus MRS Agar is a selective culture medium specifically designed for the growth and isolation of Lactobacillus species, which are important bacteria used in food fermentation and probiotic applications.

Lactobacillus MRS Agar (MRS Agar) is Recommended for the isolation and enumeration of lactic acid bacteria from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 1995, Draft ISO/DIS 13720:2010. Peptone, HM Extract B, yeast extract, and dextrose provide carbon and nitrogen sources for lactobacilli growth. Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms. Magnesium sulphate and manganese sulphate provide essential ions for the multiplication of lactobacilli. Phosphates provide good buffering action in the media. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.

Lactobacillus MRS Agar (MRS Agar)

Ingredients (Gms / Litre)

• Protease peptone: 10.000
• HM Peptone B #: 10.000
• Yeast extract: 5.000
• Dextrose (Glucose): 20.000
• Tween 80 (Polysorbate 80): 1.000
• Ammonium citrate: 2.000
• Sodium acetate: 5.000
• Magnesium sulphate: 0.100
• Manganese sulphate: 0.050
• Dipotassium hydrogen phosphate: 2.000
• Agar: 12.000
• Final pH ( at 25°C): 6.5±0.2
• **Formula adjusted, standardized to suit performance parameters
• # Equivalent to Beef extract

Directions of Lactobacillus MRS Agar

Suspend 67.15 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Click for the Data Sheet.