- Brand: Himedia
- Catalog No. M1991I
- Pack Size: 100gm
HiCrome® Chromogenic Coliform agar (CCA Agar)
-5% OffOriginal price was: 20,000.00৳ .19,000.00৳ Current price is: 19,000.00৳ .
Description
Composition**
ISO 9308-1:2014 Specification – Chromogenic Coliform agar (CCA Agar) M1991I – HiCrome®Â® Chromogenic Coliform agar (CCA Agar)
| Ingredients | g / L |
|---|---|
| Tryptone # | 1.000 |
| Yeast extract | 2.000 |
| Sodium chloride | 5.000 |
| Sodium dihydrogen phosphate, 2H2O | 2.200 |
| Disodium hydrogen phosphate | 2.700 |
| Sodium pyruvate | 1.000 |
| Sorbitol | 1.000 |
| Tryptophan | 1.000 |
| Tergitol-7 | 0.150 |
| 6-chloro-3-indoxyl β-D-galactopyranoside | 0.200 |
| 5-bromo-4-chloro-3-indoxyl- β-D-glucuronic acid cyclohexyl ammonium salt, monohydrate (X-beta-G-glucuronide CHX salt) | 0.100 |
| IPTG (Isopropyl-β-D-thiogalactopyranoside) | 0.100 |
| Agar | 15.000 |
Final pH ( at 25°C): 6.8±0.2
**Formula adjusted, standardized to suit performance parameters # Enzymatic digest of casein
Directions
Suspend 30.92 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. DO NOT OVERHEAT. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
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