N,N′-Methylenebisacrylamide powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

N,N′-Methylenebisacrylamide powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

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Original price was: 8,500.00৳ .Current price is: 7,500.00৳ .

  • Brand:             Sigma
  • Catalog No.    M7279-25G
  • Pack Size:      25G

  • Properties

    grade

    Molecular Biology

    Quality Segment

    200

    assay

    ≥99.5%

    form

    powder

    technique(s)

    electrophoresis: suitable

    impurities

    ≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

    conductivity

    ≤10 μmho (2% solution)

    anion traces

    chloride (Cl–): ≤50 mg/kg

    cation traces

    Ca: ≤10 ppm, Cd: ≤5 ppm, Co: ≤5 ppm, Cr: ≤5 ppm, Cu: ≤5 ppm, Fe: ≤5 ppm, K: ≤50 ppm, Mg: ≤5 ppm, Mn: ≤5 ppm, Na: ≤50 ppm, Ni: ≤5 ppm, Pb: ≤5 ppm, Zn: ≤5 ppm

    absorption

    ≤0.2 at 290 at 1%

    foreign activity

    DNase and RNase, none detected

    SMILES string

    C=CC(=O)NCNC(=O)C=C

    InChI

    1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

    InChI key

    ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Description

Description

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.
N,N′-Methylenebisacrylamide has been used:

  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix[1]
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

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N,N′-Methylenebisacrylamide powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

Original price was: 8,500.00৳ .Current price is: 7,500.00৳ .